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The staircase scene from Labyrinth-ya know, when David Bowie is singing "Within You".
The Holy Hand Grenade scene in Monty Python and the Holy Grail.
As mentioned earlier, the scene in Breakfast at Tiffany's where she finds Cat.
The bar scene in Airplane!
For my anime nut side, the scene in Grave of the Fireflies where Setsuko is hallucinating and Seita realizes that she's dying. I start bawling every time!
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